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KMID : 1134820200490030219
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Journal of the Korean Society of Food Science and Nutrition
2020 Volume.49 No. 3 p.219 ~ p.228
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Cardioprotective Effects of Extracted Apple Polysaccharides on Myocardial Injury in Rats
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Lim Sun-Ha
Lee Jong-won
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Abstract
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A process of directly separating the components of hot water extracted apples (HWEA) was performed to determine the polysaccharide components of apples with cardioprotective effects. Previous studies showed that HWEA and the representative polysaccharides of apples reduce the level of apoptosis and prevent cardiomyocyte death. The efficacy of pectin and xyloglucan isolated directly from apples was confirmed by comparing with purchased apple pectin and tamarind-derived xyloglucan. HWEA was used to separate pectin and xyloglucan from apples using CDTA, Na2CO3, and KOH solutions. Extracted apple pectin (EAP) and xyloglucan (EAXG) were applied at 100 mg/kg/d to the same heart model using rats, and the data were compared with the results of previously commercial apple pectin and tamarind xyloglucan. The rats were supplemented with these apple polysaccharides at a dose of 100 mg/kg/d for three days and subjected to ischemia (30 min)/reperfusion (3 h) through the ligation and release of the left anterior descending coronary artery. The cardioprotective effects of EAP and EAXG were attributed to inhibition of the apoptotic cascade by the removal of reactive oxygen species, decreased ratio of the Bax/Bcl-2 protein, and reduced generation of nicked DNA. These results suggest that supplementation of apple polysaccharides can reduce myocardial infarction by inhibiting apoptosis. In addition, this study demonstrates the feasibility of developing apple polysaccharides for cardioprotection.
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KEYWORD
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apple polysaccharide, myocardial infarction, apple pectin, apple xyloglucan, apoptosis
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